目的 研究长白山区药用植物刺五加茎中苷类化学成分。方法 利用正、反相色谱等分离手段对刺五加提取液进行分离纯化,根据波谱学方法对化合物的结构进行鉴定。结果 从刺五加中分离确定了7个化合物,分别为紫丁香苷(1),刺五加苷 E(2),cirtrusin A(3),cirtrusin B(4),丁香树脂酚葡萄糖苷(5),(-)-guaiacylglycerol-β-O-4′-coniferyl erther(6),hedyolisol(7)。结论 化合物3、4、6和7均为首次在该植物中分离得到,化合物1～5为苷类物质,并且发现化合物1～5均具有较好的自由基清除活性,其对自由基DPPH的IC50值分别为0.04、3.49、10.35、14.19和25.92 μg·mL-1。
Abstract
OBJECTIVE To study the chemical constituents in the stems of Acanthopanax senticosus from Changbai Mountain area. METHODS The chromatography on silica gel column and reverse phase column and HPLC were use to isolate and purify the constituents and the structures of the constituents were elucidated on the basis of physicochemical properties and spectral data. RESULTS Seven compounds were obtained and identitied as syringin(1),eleutheroside E(2), cirtrusin A(3), cirtrusin B(4), syringaresinol-4-O-β-D-glucopyranoside(5),(-)-guaiacylglycerol-β-O-4′-coniferyl ether(6) and hedyolisol(7). CONCULSION Compounds 3, 4, 6 and 7 are all obtained from Acanthopanax senticosus for the first time. Compounds 1-5 are identified as glycosides, showing better activity toward DPPH radical with IC50 values of 0.04, 3.49, 10.35, 14.19 and 25.92 μg·mL-1, respectively.
关键词
刺五加 /
紫丁香苷 /
刺五加苷 E /
丁香树脂酚葡萄糖苷 /
自由基清除活性
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Key words
Acanthopanax senticosus /
syringin /
eleutheroside E /
syringaresinol-4-O-β-D-glucopyranoside /
free radical scavenging activity
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中图分类号：
R284
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参考文献
[1] PAN H F,LI Y R. Study on the roots and stems fingerprinting of Acanthopanax senticosus Harms by HPLC [J]. Chin Pharm J(中国药学杂志),2010, 45(15):1136-1138.[2] NI N, LIU X Q. Advances in studies on plants of Acanthopanax Miq. in araliaceae [J]. Chin Tradit Herb Drugs (中草药), 2006, 37(12): 1895-1900.[3] ZHANG Y, LIU B Z, PEI Y H. Pharmacological action of Acanthopanax senticosus(Rupr. et Maxim.)Harms [J]. J Shenyang Pharm Univ (沈阳药科大学学报),2002, 19(2): 143-146.[4] YAN Z W. Studies on the chemical constituents and pharmacological activities of the pulp of Acanthopanax senticosus(Rupr. et Maxim.)Harms [D]. Jilin:Univ of JIlin, 2011.[5] YUAN Z,ZHOU B Y,LI X. NMR Study of citrusin A [J]. Chin J Magnet Res (波谱学杂志), 2002,19(3): 309-313.[6] LIN S, ZHANG Z X, SHEN Y H, et al. One new lignan glycoside from whole plants of Senecio chrysanthemoides [J]. China J Chin Mater Med(中国中药杂志), 2006, 36(13): 1755-1762.[7] WANG X Y,FENG X,WANG M, et al. Studies on the chemical constituents of Salicornia europaea [J]. J Chin Med Mater(中药材), 2011, 34(1): 67-69.[8] WANG Y, ZHANG H N, WANG W J, et al. Chemical constituents from barks of Ailanthus altissima [J]. Chin Tradit Herb Drugs (中草药), 2012, 43(4): 649-652.[9] XIONG L, ZHU C G, LI Y R, et al. Lignans and neolignans from Sinocalamus affinis and their absolute configurations [J]. J Nat Prod, 2011, 74(10): 1188-1200.
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脚注
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基金
国家自然科学基金资助项目(20962020/B0204)
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